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Preparing qualified and excellent HE slices is a prerequisite for correct diagnosis in clinicopathological work. In the process of making HE slices, different objective factors such as climate, slice material, dyeing reagent and tissue particularity often influence the results of making HE slices. Some tips can improve the quality of production.
When the weather is dry in winter or in the north and the temperature is low, it is difficult to make pathological section. When cutting, breathe a few hot breaths on the tissue block. When cutting again, the section will roll up naturally, and then expand the section in warm water. This can basically solve the problem. To exhale a few hot breaths on the tissue, the main purpose is to eliminate the static electricity on the surface of pathological sections. At the same time, it is better to thinner the micron on the microtome, usually to 2-3 micron, but some difficult tissues (such as lung tissue) still need 4-5 micron. Otherwise, the slices are thicker, which is unfavorable for lymphoid tissue observation.
It is very important to have a good Microtome Blades for making tissue slices. We should choose a Microtome Blades with strong toughness and sharp edge.
In routine pathological section work, if there are not many lung tissue specimens, there are few special methods to deal with these specimens. Because the lung tissue is loose and the alveoli contain air, the air can be removed by adding negative pressure in the process of fixation, dehydration, transparency and immersion, which is very helpful to improve the quality of sections. Like other tissues, the key of lung tissue is fixation and dehydration. The first way is to dehydrate the lung tissue adequately with low concentration alcohol, but the time can not be too long, too long will make the tissue brittle.
The main purpose of pathological tissue fixation is to prevent autolysis and degeneration of tissues. Most of the clinically common pathological specimens sent for examination are large specimens, small containers and few fixatives, which can not guarantee good tissue fixation. After routine sampling, the specimens were re-immobilized in 65%-70% ethanol solution containing 10% formalin. This re-immobilization has both immobilization and dehydration effects, enhances tissue hardness, facilitates slicing, and overcomes the gray phenomenon of nuclear staining.
The melting point of paraffin is 56 ~58 C. The temperature requirement of thermostat is 2 ~4 C higher than that of paraffin melting point. The temperature should not be too high. Especially when embedding, the structure can not be directly heated by flame, which makes the structure brittle. Embedding wax must be dissolved and mixed with old and new wax. In this way, the wax stickiness can be enhanced, and the connection of tissue sections can be better.
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